Mapping by multifragment cloning in vivo.
other · Level V
Where this comes from
- Record sourced from PubMed, PMID 8524832.
- Also identified by PMC identifier 40470.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes.
Medical subject headings
- Bacterial Proteins
- Chromosome Mapping
- Cloning, Molecular
- Escherichia coli
- Point Mutation