Z-DNA-forming sites within the human beta-globin gene cluster.
basic_science · Level V
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- Record sourced from PubMed, PMID 8570634.
- Also identified by PMC identifier 40132.
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Abstract
Agarose-encapsulated, metabolically active, permeabilized nuclei from human hematopoietic cell lines were tested for Z-DNA formation in the beta-globin gene cluster. Biotinylated monoclonal antibodies against Z-DNA were diffused into the nuclei and cross-linked to DNA with a 10-ns laser exposure at 266 nm. Following digestion with restriction enzymes, fragments that had formed Z-DNA were isolated. Seventeen regions with Z-DNA sequence motifs in the 73-kb region were studied by PCR amplification, and five were found in the Z conformation.
Medical subject headings
- DNA
- Globins
- Multigene Family
- Nucleic Acid Conformation