In vivo examination of membrane protein localization and degradation with green fluorescent protein.
basic_science · Level V
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- Record sourced from PubMed, PMID 8570643.
- Also identified by PMC identifier 40142.
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Abstract
To test the utility of green fluorescent protein (GFP) as an in vivo reporter protein when fused to a membrane domain, we made a fusion protein between yeast hydroxymethylglutaryl-CoA reductase and GFP. Fusion proteins displayed spatial localization and regulated degradation consistent with the native hydroxymethylglutaryl-CoA reductase proteins. Thus, GFP should be useful in the study of both membrane protein localization and protein degradation in vivo.
Medical subject headings
- Cell Compartmentation
- Genes, Reporter
- Hydroxymethylglutaryl CoA Reductases
- Luminescent Proteins
- Membrane Proteins