Intraocular levels of methotrexate after intravenous administration.

Abstract

To determine if adequate intraocular levels of methotrexate are achieved after intravenous administration. After intravenous administration, methotrexate levels were determined in the serum, the anterior chamber, and the cerebrospinal fluids of a patient with recurrent ocular lymphoma. A fluorescence polarization immunoassay was used to make the determinations. At seven hours into a 24-hour intravenous infusion, methotrexate was at cytotoxic level in all samples. At 74 hours, cytotoxic levels were present only in the aqueous humor. Sustained cytotoxic ocular methotrexate levels are achievable after systemic administration.