Genetic evidence that the RAG1 protein directly participates in V(D)J recombination through substrate recognition.
basic_science · Level V
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- Record sourced from PubMed, PMID 8637873.
- Also identified by PMC identifier 39796.
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Abstract
RAG1 protein is essential for the activation of V(D)J recombination in developing lymphocytes (V, variable; D, diversity; J, joining). However, it has not been determined whether its role involves substrate recognition and catalysis. A single amino acid substitution mutation in the RAG1 gene has now been identified that renders its activity sensitive to the sequence of the coding region abutting the heptamer site in the recombination signal sequence. These results strongly imply that RAG1 interacts directly with DNA.
Medical subject headings
- Gene Rearrangement, B-Lymphocyte
- Homeodomain Proteins
- Proteins
- Recombination, Genetic