Overexpression of cyclin E in the HC11 mouse mammary epithelial cell line is associated with growth inhibition and increased expression of p27(Kip1).

Abstract

To elucidate the role of cyclin E in cell growth and tumorigenesis in mammary epithelial cells, we have used retrovirus-mediated transduction to generate derivatives of the nontransformed HC11 mouse mammary epithelial cell line that stably express a human cyclin E cDNA (HU4). These derivatives expressed two distinct forms of the exogenous cyclin E protein, which were about M(r) 50,000 and M(r) 42,000, thus corresponding to endogenous cyclin E proteins found in human cells. In contrast to results obtained previously in fibroblasts, overexpression of the HU4 cyclin E cDNA in HC11 cells was associated with an increase in cell size, lengthening of G(1), and inhibition of both anchorage-dependent and independent growth. Furthermore, when quiescent serum-starved cells were restimulated with serum, entry into the S-phase was delayed in the overexpressor cells. Under these conditions, there was also delayed induction in the expression of the endogenous cyclin E protein and in other events involved in the G(1) transition. Despite the high level of expression of the exogenous cyclin E, the derivatives did not display increased cyclin E-associated in vitro kinase activity. The HC11 cells that overexpressed the exogenous cyclin E displayed an increase in the cyclin/cyclin-dependent kinase inhibitor p27(Kip1) in both asynchronous exponentially dividing and synchronous cell populations. These findings indicate that increased expression of this cyclin E cDNA in HC11 cells inhibits rather than stimulates growth and that this may be due to increased expression of the inhibitor p27(Kip1).