Crystal structure of a human TATA box-binding protein/TATA element complex.

Nikolov, D B; Chen, H; Halay, E D; Hoffman, A; Roeder, R G; Burley, S K · Proc Natl Acad Sci U S A · 1996

basic_science · Level V

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Abstract

The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstroms resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9-angstroms resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box.

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