Separation of the transcriptional coactivator and antirepression functions of transcription factor IIA.
basic_science · Level V
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- Record sourced from PubMed, PMID 8692860.
- Also identified by PMC identifier 39068.
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Abstract
Human transcription factor IIA (TFIIA) is composed of three subunits (alpha, beta, and gamma). TFIIA interacts with the TATA-box binding protein and can overcome repression of transcription. TFIIA was found to be necessary for VP16-mediated transcriptional activation through a coactivator function. We have separated the coactivator and antirepression activities of TFIIA. A TFIIA lacking the alpha subunit was isolated from HeLa cells. This "mini-TFIIA" interacts with the TATA-box binding protein and can overcome repression of transcription, but it is defective in transcriptional coactivator function.
Medical subject headings
- Repressor Proteins
- Trans-Activators
- Transcription Factors