Design of artificial sequence-specific DNA bending ligands.
basic_science · Level V
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- Record sourced from PubMed, PMID 8790361.
- Also identified by PMC identifier 38459.
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Abstract
Proteins that bend DNA are important regulators of biological processes. Sequence-specific DNA bending ligands have been designed that bind two noncontiguous sites in the major groove and induce a bend in the DNA. An oligonucleotide containing pyrimidine segments separated by a central variable linker domain simultaneously binds by triple helix formation two 15-bp purine tracts separated by 10 bp. Bend angles of 61 degrees, 50 degrees, and 38 degrees directed towards the minor groove were quantitated by phasing analysis for linkers of four, five, and six T residues, respectively. The design and synthesis of nonnatural architectural factors may provide a new class of reagents for use in biology and human medicine.
Medical subject headings
- Cross-Linking Reagents
- DNA
- Drug Design
- Nucleic Acid Conformation
- Oligonucleotides