Parallel human genome analysis: microarray-based expression monitoring of 1000 genes.
basic_science · Level V
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- Record sourced from PubMed, PMID 8855227.
- Also identified by PMC identifier 38202.
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Abstract
Microarrays containing 1046 human cDNAs of unknown sequence were printed on glass with high-speed robotics. These 1.0-cm2 DNA "chips" were used to quantitatively monitor differential expression of the cognate human genes using a highly sensitive two-color hybridization assay. Array elements that displayed differential expression patterns under given experimental conditions were characterized by sequencing. The identification of known and novel heat shock and phorbol ester-regulated genes in human T cells demonstrates the sensitivity of the assay. Parallel gene analysis with microarrays provides a rapid and efficient method for large-scale human gene discovery.
Medical subject headings
- DNA, Complementary
- Gene Expression
- Gene Library
- Heat-Shock Response
- Nucleic Acid Hybridization
- T-Lymphocytes
- Tetradecanoylphorbol Acetate