Inhibition of transport processes of intestinal segments following augmentation enterocystoplasty in rats.

Abstract

Sodium and chloride transport was measured by using the Ussing chamber-voltage clamp technique to evaluate alterations occurring in ileal segments interposed in the urinary tract in a rat enterocystoplasty model. In adult male Sprague-Dawley rats which had undergone augmentation ileocystoplasties 3 months earlier, intestinal segments were explanted from the neobladders and native ileal segments were removed separately for electrolyte transport and morphometric analysis. Basal ileal active sodium and chloride absorption and cyclic nucleotide-induced chloride secretion were attenuated in transplanted ileum in contrast to control tissue. Morphologically, there was villus epithelial hyperplasia, crypt hyperplasia and chronic inflammation in transplanted segments, but villus height:crypt length ratios were unchanged. Pathophysiologic derangements in intestinal segments interposed in the urinary tract include inhibition of sodium and chloride transport processes and mucosal hyperplasia. The use of the Ussing chamber/voltage clamp technique described herein offers a new approach for additional studies of the metabolic consequences following urinary diversion.