Comparison of arterial and venous patency in a rat model of subendothelium-stimulated thrombosis.

Abstract

The effectiveness of anticoagulants and platelet aggregation inhibitors was compared using comparable rat models of arterial and venous thrombosis. A mechanical endothelium-denuding injury was created on the lumenal surface of donor Lewis rat carotid arteries. These were cut into 4-5 mm lengths and grafted into femoral veins and arteries of recipient syngeneic rats using microvascular anastomotic techniques. Recipients received either systemic heparin, or aspirin with dipyridamole, or saline (control). In the arteries, the 1-day patency rate was 94% in the heparin-treated rats, but only 50% in the aspirin/dipyridamole group and 44% in the control group. The venous patency rate was 56% in the heparin group, 31% in the aspirin/dipyridamole group, and 0% in the control group. This unique model for comparing thrombosis in arteries and veins shows that anticoagulation is more effective than inhibition of platelet aggregation in the rat arterial system, with less of a differential effect in the venous system.