Evidence for a mediator cycle at the initiation of transcription.
basic_science · Level V
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- Record sourced from PubMed, PMID 9177171.
- Also identified by PMC identifier 21003.
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Abstract
Free and elongating (DNA-bound) forms of RNA polymerase II were separated from yeast. Most cellular polymerase II was found in the elongating fraction, which contained all enzyme phosphorylated on the C-terminal domain and none of the 15-subunit mediator of transcriptional regulation. These and other findings suggest that mediator enters and leaves initiation complexes during every round of transcription, in a process that may be coupled to C-terminal domain phosphorylation.
Medical subject headings
- RNA Polymerase II
- Transcription Factors
- Transcription Factors, General
- Transcription Factors, TFII
- Transcription, Genetic
- Transcriptional Elongation Factors