Evidence for a role of CRM1 in signal-mediated nuclear protein export.
basic_science · Level V
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Abstract
Chromosome maintenance region 1 (CRM1), a protein that shares sequence similarities with the karyopherin beta family of proteins involved in nuclear import pathway, was shown to form a complex with the leucine-rich nuclear export signal (NES). This interaction was inhibited by leptomycin B, a drug that prevents the function of the CRM1 protein in yeast. To analyze the role of the CRM1-NES interaction in nuclear export, a transport assay based on semipermeabilized cells was developed. In this system, which reconstituted NES-, cytosol-, and energy-dependent nuclear export, leptomycin B specifically blocked export of NES-containing proteins. Thus, the CRM1 protein could act as a NES receptor involved in nuclear protein export.
Medical subject headings
- Carrier Proteins
- Cell Nucleus
- DNA-Binding Proteins
- I-kappa B Proteins
- Karyopherins
- Nuclear Proteins
- Protein Sorting Signals
- Receptors, Cytoplasmic and Nuclear