Discrete start sites for DNA synthesis in the yeast ARS1 origin.
basic_science · Level V
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Abstract
Sites of DNA synthesis initiation have been detected at the nucleotide level in a yeast origin of bidirectional replication with the use of replication initiation point mapping. The ARS1 origin of Saccharomyces cerevisiae showed a transition from discontinuous to continuous DNA synthesis in an 18-base pair region (nucleotides 828 to 845) from within element B1 toward B2, adjacent to the binding site for the origin recognition complex, the putative initiator protein.
Medical subject headings
- DNA Replication
- DNA, Fungal
- DNA-Binding Proteins
- Replication Origin
- Saccharomyces cerevisiae