Probing single secretory vesicles with capillary electrophoresis.
basic_science · Level V
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- Record sourced from PubMed, PMID 9469805.
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Abstract
Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single vesicle that had attoliters (10(-18) liters) of volume was introduced into the tapered inlet of a separation capillary. Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2, 3-dicarboxaldehyde before separation. The resultant electropherograms indicated distinct variations in the contents of single vesicles.
Medical subject headings
- Amines
- Amino Acids
- Cytoplasmic Granules
- Electrophoresis, Capillary
- Taurine