The role of inducible nitric oxide synthase in the host response to Coxsackievirus myocarditis.

Zaragoza, C; Ocampo, C; Saura, M; Leppo, M; Wei, X Q; Quick, R; Moncada, S; Liew, F Y et al. · Proc Natl Acad Sci U S A · 1998

basic_science · Level V

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Abstract

The host response to Coxsackievirus infection is complex, including T lymphocytes, B lymphocytes, natural killer cells, and macrophages. Although Coxsackievirus infection induces expression of inducible nitric oxide synthase (NOS2; EC 1.14.13.39) in macrophages, the precise role of NOS2 in the host response to Coxsackievirus myocarditis has been unclear. We show, by using mice homozygous for a disrupted NOS2 allele, that Coxsackievirus replicates to higher titers in NOS2(-/-) mice, that the host lacking NOS2 clears virus more slowly than the wild-type host, and that myocarditis is much more severe in infected NOS2(-/-) mice. These data show that NOS2 is crucial for the host response to Coxsackievirus in the mouse.

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