Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast.
basic_science · Level V
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Abstract
The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the delta 22-desaturase gene and introduction of the Arabidopsis thaliana delta 7-reductase activity and coexpression of bovine side chain cleavage cytochrome P450, adrenodoxin, and adrenodoxin reductase, lead to pregnenolone biosynthesis from a simple carbon source. Following additional coexpression of human 3 beta-hydroxysteroid dehydrogenase/isomerase, pregnenolone is further metabolized to progesterone. Steroid formation appears to be coupled to yeast sterol biosynthesis.
Medical subject headings
- Genetic Engineering
- Oxidoreductases Acting on CH-CH Group Donors
- Pregnenolone
- Progesterone
- Saccharomyces cerevisiae