Comparison of virus culture and direct immunofluorescent staining of cytocentrifuged virus transport medium for detection of varicella-zoster virus in skin lesions.
case_control · Level III
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Abstract
Direct immunofluorescent staining of centrifuged viral transport medium (CVTM) was compared with conventional cell culture for the detection of varicella-zoster virus (VZV) in 87 dermal lesions from 84 patients. A total of 21 (24%) were positive for VZV; 8 (38%) of these were positive by culture and CVTM, 13 (62%) by CVTM alone, and none by culture only. Virus cultures were positive for VZV in an average of 9.1 days (range, 4-20 days). CVTM, using cytocentrifugation, is more sensitive and rapid than conventional cell culture for the detection of VZV in cutaneous specimens.
Medical subject headings
- Animals
- Cells, Cultured
- Centrifugation
- Culture Media, Conditioned
- False Negative Reactions
- Fluorescent Antibody Technique, Direct
- Herpesvirus 3, Human
- Herpesvirus 3, Human/isolation & purification
- Humans
- Macaca mulatta
- Sensitivity and Specificity
- Skin Diseases
- Skin Diseases/virology
- Time Factors