Promotion of met-tRNAiMet binding to ribosomes by yIF2, a bacterial IF2 homolog in yeast.
basic_science · Level V
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Abstract
Delivery of the initiator methionine transfer RNA (Met-tRNAiMet) to the ribosome is a key step in the initiation of protein synthesis. Previous results have indicated that this step is catalyzed by the structurally dissimilar translation factors in prokaryotes and eukaryotes-initiation factor 2 (IF2) and eukaryotic initiation factor 2 (eIF2), respectively. A bacterial IF2 homolog has been identified in both eukaryotes and archaea. By using a combination of molecular genetic and biochemical studies, the Saccharomyces cerevisiae IF2 homolog is shown to function in general translation initiation by promoting Met-tRNAiMet binding to ribosomes. Thus, the mechanism of protein synthesis in eukaryotes and prokaryotes is more similar than was previously realized.
Medical subject headings
- DNA-Binding Proteins
- Peptide Initiation Factors
- Protein Biosynthesis
- RNA, Transfer, Met
- Ribosomes
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins