Open sandwich ELISA: a novel immunoassay based on the interchain interaction of antibody variable region.
basic_science · Level V
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Abstract
We describe an immunoassay that is based on the interchain interaction of separated VL and VH chains from a single chain antibody variable region. In the presence of antigen, the chains reassociate. VL fragments of anti-hen egg lysozyme (HEL) antibody HyHEL-10 were immobilized on microtiter plates. Samples were coincubated with an M13-displayed VH chain, and assayed with peroxidase-labeled anti-M13 antibody. Signal was detected in direct proportion to the amount of HEL in the sample. Wide dynamic range with < 15 ng/ml sensitivity was attained.
Medical subject headings
- Enzyme-Linked Immunosorbent Assay
- Immunoglobulin Fragments
- Immunoglobulin Variable Region
- Muramidase