The SbcCD nuclease of Escherichia coli is a structural maintenance of chromosomes (SMC) family protein that cleaves hairpin DNA.
basic_science · Level V
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- Record sourced from PubMed, PMID 9653124.
- Also identified by PMC identifier 20913.
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Abstract
Hairpin structures can inhibit DNA replication and are intermediates in certain recombination reactions. We have shown that the purified SbcCD protein of Escherichia coli cleaves a DNA hairpin. This cleavage does not require the presence of a free (3' or 5') DNA end and generates products with 3'-hydroxyl and 5'-phosphate termini. Electron microscopy of SbcCD has revealed the "head-rod-tail" structure predicted for the SMC (structural maintenance of chromosomes) family of proteins, of which SbcC is a member. This work provides evidence consistent with the proposal that SbcCD cleaves hairpin structures that halt the progress of the replication fork, allowing homologous recombination to restore DNA replication.
Medical subject headings
- Bacterial Proteins
- DNA Replication
- DNA, Bacterial
- Deoxyribonucleases
- Escherichia coli
- Escherichia coli Proteins
- Exonucleases
- Nuclear Proteins