A novel DNA-binding motif in MarA: the first structure for an AraC family transcriptional activator.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 9724717.
- Also identified by PMC identifier 27908.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
A crystal structure for a member of the AraC prokaryotic transcriptional activator family, MarA, in complex with its cognate DNA-binding site is described. MarA consists of two similar subdomains, each containing a helix-turn-helix DNA-binding motif. The two recognition helices of the motifs are inserted into adjacent major groove segments on the same face of the DNA but are separated by only 27 A thereby bending the DNA by approximately 35 degrees. Extensive interactions between the recognition helices and the DNA major groove provide the sequence specificity.
Medical subject headings
- Bacterial Proteins
- DNA-Binding Proteins
- Escherichia coli Proteins
- Repressor Proteins
- Trans-Activators
- Transcription Factors