Protein phosphatase 2A is required for the initiation of chromosomal DNA replication.
basic_science · Level V
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- Record sourced from PubMed, PMID 9843951.
- Also identified by PMC identifier 24511.
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Abstract
Protein phosphatase 2A (PP2A) is an abundant, multifunctional serine/threonine-specific phosphatase that stimulates simian virus 40 DNA replication. The question as to whether chromosomal DNA replication also depends on PP2A was addressed by using a cell-free replication system derived from Xenopus laevis eggs. Immunodepletion of PP2A from Xenopus egg extract resulted in strong inhibition of DNA replication. PP2A was required for the initiation of replication but not for the elongation of previously engaged replication forks. Therefore, the initiation of chromosomal DNA replication depends not only on phosphorylation by protein kinases but also on dephosphorylation by PP2A.
Medical subject headings
- DNA Replication
- Phosphoprotein Phosphatases