A microfabricated device for sizing and sorting DNA molecules.
basic_science · Level V
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- Record sourced from PubMed, PMID 9874762.
- Also identified by PMC identifier 15083.
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Abstract
We have demonstrated a microfabricated single-molecule DNA sizing device. This device does not depend on mobility to measure molecule size, is 100 times faster than pulsed-field gel electrophoresis, and has a resolution that improves with increasing DNA length. It also requires a million times less sample than pulsed-field gel electrophoresis and has comparable resolution for large molecules. Here we describe the fabrication and use of the single-molecule DNA sizing device for sizing and sorting DNA restriction digests and ladders spanning 2-200 kbp.
Medical subject headings
- DNA, Viral
- Miniaturization
- Molecular Biology