Different growth behaviour of human umbilical vein endothelial cells and an endothelial cell line seeded on various polymer surfaces.

Abstract

Major obstacles for successful application of endothelial cell seeding in synthetic vascular grafts include the source of autologous endothelial cells, the efficiency of cell seeding and detachment of adherent endothelial cells from the graft surface after restoration of circulation. Human umbilical vein endothelial cells (HUVECs) are frequently used to investigate the in vitro adhesion and proliferation of endothelial cells on polymer surfaces. In order to minimize the biological variation of HUVECs isolated from different umbilical veins, it would be advantageous to use an endothelial cell line in in vitro proliferation experiments. Aim of the present study was to compare the proliferation of primary HUVECs and the endothelial cell line EC-RF24 on several polymer surfaces coated with various concentrations of the adhesive protein fibronectin. EC-RF24 cells grow to a higher density than primary HUVECs. Moreover, the EC-RF24 cell line is able to proliferate on surfaces with sub-optimal adhesive properties. Therefore, it is concluded that the EC-RF24 cell line is less suitable for evaluation of the in vitro proliferation of endothelial cells on polymer surfaces.